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engIsolation of high density lipoprotein subfractions by preparative free flow electrophoresis.
A new method for the isolation of human plasma high density lipoprotein (HDL) subfractions is described. After extraction with ethanol/ether and density gradient ultracentrifugation, HDL was obtained from 0.5 ml plasma, and electrophoresis was carried out at pH 8.9. Five fractions were obtained: super-fraction 1, which contained phospholipids, apoprotein A-I, and apoprotein A-II; super-fraction 2, which contained both apoproteins; and two HDL subfractions 1a and 1b, which contained mostly apoprotein A-I and A-II. The subfractions 1a and 1b had mean diameters of 4.72 +/- 0.15 and 3.23 +/- 0.07 nm, respectively (n = 10). The isolates were comparable in lipid composition to HDL isolated by gel filtration and ultracentrifugation. The technique is suitable for both qualitative and quantitative isolation of HDL subfractions.Radio frequency (RF) radio-frequency identification (RFID) systems, or other similar systems, are used to manage or monitor the flow of products, materials, or other items through an assembly line. RFID systems are generally used to track and identify articles through the assembly line. It is often useful for an assembly line to be monitored for conditions such as speed, throughput, or other such conditions. This allows a manufacturer to optimize conditions in order to produce a better product. To achieve this, it is useful for an assembly line to track and record various conditions associated with the assembly line at a particular location. Data regarding the assembly line can be used to monitor, track, and manage conditions.The exchange of heat (via the latent heat of fusion) between the solid powder and liquid carrier is one of the more important aspects for the preparation of hydrogels. This is one of the reasons why hydrogels have become an important part of the arsenal of materials used to absorb or retain and deliver drugs. There are two important limiting features for hydrogels which must be taken into consideration in the preparation and development of such hydrogels.
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